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Document Type |
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Thesis |
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Document Title |
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GENETIC DIVERSITY OF HUMAN RHINOVIRUSES (HRVS) ISOLATED FROM JEDDAH, SAUDI ARABIA التنوع الوراثي لفيروسات الرينو المعزولة من جدة، بالمملكة العربية السعودي ة |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Human Rhinoviruses (HRVs) are the most common Viral Respiratory Tract Infections (VRTI) and the primary cause of the common cold. It can cause serious complication upon infection, especially to people who are prone to complication. HRVs include three species A, B and C joining are approximately 160 known subtypes. In Saudi Arabia, the rate of infection is raising due to some influencing factors. In this research we described the genetic characteristic of HRVs by laboratory techniques that will help in the future in giving a bigger image on how to resist this virus to maintain the general health of the public. In this study, A total of 34 clinical HRVs positive samples was subjected to RNA extraction, products were revers transcribed to cDNA using SuperScriptTM ׀׀׀ Reverse Transcriptase, followed by PCR amplification using particular primers for first and nested PCR. The amplified products were analyzed and purified, then sequenced on ABI 3500 Genetic Analyzer sequencer. Genome assembly and phylogenetic analysis was performed using Geneious R09 software and MEGA11.The occurrence of HRVs types in this study presented as HRV-A was the most common followed by HRV-C lastly HRV-B (44.11%, 41.17% and 14.7% respectively), HRV-A and C were the predominant as similar to previous studies. Genetic analysis revealed 10 HRV-A subtypes, 3 HRV-B and 14 HRV-C among 34 HRVs samples, where most of the HRVs subtypes clustered with USA, China and Australia. The change in genotype requires constant surveillance for genetic characterization of HRVs to guide for the implementation of preventive strategies to maintain the general health of the public. |
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Supervisor |
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Dr. Lina Hussein Misri Bagri |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Added Date |
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Monday, September 18, 2023 |
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Researchers
| اسراء حسن الحربي | Alharbi, Israa Hassan | Researcher | Master | |
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