Document Details
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Document Type |
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Thesis |
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Document Title |
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FABRICATION OF FILMS FROM POLYMER NANOCOMPOSITE AS ANTIMICROBIAL, ANTICANCER AND WOUND HEALING AGENTS تصنيع أفلام من بوليمرات متناهية الصغر كعوامل مضاده للميكروبات والسرطانات ولالتئام الجروح |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Resistance to antibiotics is increased in all hospitals and bacterial pathogens that contaminate burns and wounds are spread. Their resistance to well-known antibiotics is recorded and every day resistant isolates were counted and studied. The present study aimed to prepare new polymers with antimicrobial activities. In the present research the effect of incorporating of three polymers chitosan, PVP, and collagen on antimicrobial, antibiofilm formation, antitumor, wound healing, mechanical, physical and morphological properties of nanocomposite film was investigated. The effect of some polymers prepared from chitosan, Polyvinylpyrrolidone (PVP) and collagen associated with nanoparticles of Cu+, Ti4+, and Ag+ was determined using some bacterial pathogens as test organisms. The tested bacteria were two gram negative, Pseudomonas aeruginosa and Escherichia coli and four gram positive, Enterococcus faecalis, Streptococcus sp., Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA). The antimicrobial activity of the prepared polymers was determined using three methods; diffusion method, colony forming unit (CFU) and Optical density method. The Beetroot, Curcuma and Red cabbage plant were extracted to obtain the extract which was used to prepare the films. The results showed that the all nanocomposite films were active against different pathogenic bacteria. The most active film was chitosan-PVP-Collagen nanocomposite with three different metals. In diffusion method Gram negative Pseudomonas aeruginosa was the most sensitive bacteria to the incorporating film with three nanoparticles with inhibition zone 17.30.05 mm and gram-positive E. faecalis was the most sensitive bacteria to blend film with TiO2 with inhibition zone (17.3and 32mm respectively). Reductions in biofilm viable cell numbers ranging from 0.0% to 91.0% were demonstrated for tested bacteria in comparison to controls. Colon carcinoma was used to investigate the potency of chitosan, PVP, collagen nanocomposite solution as antitumor activity. The result showed high activity by reduction the number of treated cells to 24.72±0.99%. The blends of chitosan, PVP, collagen and nanoparticles were investigated by Attenuated total reflection Fourier transform infrared (FTIR) spectroscopy. The size and distribution of the CuO, TiO2 and Ag nanoparticles were measured using scanning electron microscope. The prepared nanocomposite dressing has excellent as a wound healing in tested rats. Compared to group 1 (negative control) no treatment, group 2 treated with chitosan, PVP, collagen films and group 3 treated with chitosan, PVP and collagen nanocomposite films, the prepared nano dressing films caused an accelerated healing of open excision type wounds in albino rat model. The synergistic between polymers and nanoparticles in dressing material like good antibacterial ability, wound appearance and wound closure rate through in vivo test makes it a suitable candidate for wound healing applications. In conclusion, films of chitosan, PVP, collagen and nanoparticles (CuO, TiO2, AgNO3) had excellent antibacterial ability, antibiofilm, antitumor and wound dressing. |
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Supervisor |
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Prof. Dr. Magda M. Aly |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Tuesday, June 23, 2020 |
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Researchers
| مشاعل أحمد الغامدي | Alghamdi, Mashail Ahmad | Researcher | Doctorate | |
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