Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
Biodegradation of Petroleum Hydrocarbons in Seawater and Bacterial Communities Associated with Degradation التحلل الحيوي للهيدروكربونات البترولية في مياه البحر والمجتمعات البكتيرية المرتبطة بالتحلل |
|
Subject |
: |
Faculty of Marine Sciences |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
The present study, water samples were collected from the boat fuel filling station, Abhour, Jeddah, Saudi Arabia and were enriched in MSM. Biodegradation by halophilic bacterial consortium of LMW PAHs, such as PHE, and FLU, and HMW of Alkanes were studied for HXD, as the sole source of carbon in mineral salts medium (MSM) at different concentrations of PAHs (50,100,200 and 500) mg/L, and different concentrations of HXD (0.5,1 and 1.5(v/v %)) , under saline condition (40 g/L NaCl concentrations). The recorded results were a complete degradation of PHE at 50 and 100 mg/L concentration, FLU also at 100 and 200 mg/L concentration. PHE concentration at 200 and 500 mg/L, the results of the degradation were 97% and 96% at 8 and 12 days respectively, and FLU at 500 mg/L concentration. The results of complete degradation of HXD were recorded at 0.5 and 1 (v/v) % concentration in 4 and 6 days, while at 1.5 % concentration the degradation rate was 88 % in 10 days. The treatment performance of the halophilic bacterial consortium was examined in CSTR under halophilic condition, the result recorded 93% COD removal in 40th day. The LMW PAHs were completely degraded 10 days, whereas HMW PAHs showed 92% degradation in 40 days was recorded under halophilic condition. Phylogenetic analysis revealed the bacterial strains present in the consortium (PHE) was dominated by Propionispira followed by Martelella, while HXD was dominated by Bacillus followed by Martelella and Microbacterium. The samples from CSTR revealed of archae group and Bacterial strains. Archael strains such as Candidatus Nitrosocosmicus, Methanosaeta sp, Methanobrevibacter smithii and Uncultured archaeon were present in the consortium. The bacterial strains such as Martelella, followed by Propionispira, Bacillus. Thus, halophilic consortium can be a promising technology for removing hydrocarbon contaminants present in marine aquatic environments. |
|
Supervisor |
: |
Dr. Mamdoh Taha Jamal |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1441 AH
2020 AD |
|
Co-Supervisor |
: |
Dr. Arulazhagan Pugazhendi |
|
Added Date |
: |
Tuesday, June 9, 2020 |
|
Researchers
| رمزي حيدر عمران | Amran, Ramzi Haidar | Researcher | Master | |
|
Back To Researches Page
|