Document Details
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Document Type |
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Thesis |
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Document Title |
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Flavonoids of Capparis Cartilaginea Fruit Extract Effect on Wound Healing in Human Prostate Cancer Cell Line تأثير الفلافونويدات المستخلصة من ثمرة نبات الكبر الغضروفي على التأم الجروح في خلايا البروستاتا البشرية السرطانية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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In the developed countries, prostate cancer (PCa) is the most common cancer in males and its mortality is just beyond the lung and colorectal cancer. The prevalence of PCa varies nationwide, and the percentage of cases differs by more than 25-fold globally. Medical treatment for the advanced-stage PCa is still developing, even with the recent progress in surgery, radiation therapy, hormonal therapy, and medical management. Alternatively, several dietary cancer chemo-preventive agents could offer other approaches to decreasing the incidence of PCa. Various therapies have been proposed for cancer treatment, many of which use products derived from plants. Plants are a reservoir of natural chemicals that may offer chemoprotective ability toward cancer. Epidemiologic studies that focused on the Asian population, presented a protective effect of high phytoestrogen diets which decrease the incidence of some hormone-responsive cancers, such as breast and PCa. Plant phytoestrogens consist of flavonoids, coumestans, and lignans. Phytoestrogens such as genistein, resveratrol, and quercetin broadly found in human and animal diets. It possesses chemo-preventive characteristics against estrogen-responsive diseases such as inhibition of tumor cell growth and reducing serum cholesterol. Active compounds of Capparis Cartilaginea (C. cartilaginea) were found in nearly all plants of Capparidaceae family such as alkaloids, tannins, saponins, steroids, terpenoids, flavonoids, phloatunnins, phenolic constituents, and cardic glycosides. The present study aimed to investigate the cytotoxic effect of ethanol extract of the C. cartilaginea fruit on the 22RV1 human prostate cancer cell line. The Gas chromatography-mass spectrometry analysis (GC-MS) was used to highlight the main constituents of C. cartilaginea fruit extracts. Morphological alteration and migratory capacity of the cell after exposure to ethanol extract of C. cartilaginea fruit were observed under an inverted light microscope.in a dose and time-dependent manner. Bioinformatics analysis was used to discover new gene targets. The cytotoxicity of ethanol extract was carried out on the 22RV1 cells by 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay (MTT). The extract was added at different concentrations (0.5, 5, 10, 15 and 20 μg/ml). The level of cytotoxicity was determined by calculating the percentage of the viable cell after 24, 48, and 72hours (hr) treatment with the extract. Cell morphological changes were observed under an inverted light microscope. The effect of C. cartilaginea fruit extract on cell migration was evaluated by in vitro wound-healing scratch assay. GC chromatogram profile determined the existence of six main peaks and related components were known as 2-Furancarboxaldehyde, 5-methyl-; 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-; 5-Hydroxymethylfurfural; Hexadecanoic acid, ethyl ester; 9-Octadecenamide, (Z)-; and 13-Docosenamide, (Z)-. The MTT assay showed that C. cartilaginea fruit extract exhibited a significant cytotoxic effect on 22RV1 cells after 72hr treatment with a value of (0.088±0.031) μg/ml. Morphological alteration of the cell after exposure to C. cartilaginea fruit extract revealed that the C. cartilaginea fruit extract had an outstanding effect on cell death, and the mortality increased correspondingly with the concentration increment. Bioinformatics results reveled that SCGB3A1 and THBS4 genes besides several other genes were associated with cell migration. In future studies, we suggest the use of other parts of the C. cartilaginea plant such as the stem, roots, and leaves on human normal prostate and prostate cancer cells. Also, study the effect of the C. cartilaginea fruit extract on SCGB3A1 and THBS4 expression. The results suggested the possible use of ethanol extract of C. cartilaginea fruit for preparing herbal medicine for the cancer-related disease. |
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Supervisor |
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Dr. Hala Salim Sonbol |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Added Date |
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Saturday, June 6, 2020 |
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Researchers
| مرام بكر هوساوي | Hosawi, Maram Bakr | Researcher | Master | |
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