Document Details
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Document Type |
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Thesis |
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Document Title |
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GENETIC STUDIES ON THE POTENTIAL EFFECT OF VITAMIN A AS AN ANTIOXIDANT AGENT ASSOCIATED WITH CANCER ILLNESSES IN MICE (MUS MUSCULUS) دراسات وراثية على التأثير المحتمل لفيتامين أ كعامل مضاد للأكسدة المرتبط بمرض السرطان فى الفئران |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Vitamin A has become widely available in drug stores and it may be taken for a variety of reasons. In recent years, epidemiological studies demonstrated the effectiveness of vitamin A on preventing some types of cancer. On the other hand, there are contra dietary evidences regarding its safety in some cancer cases. Therefore, the present study was aimed to highlight the potential effect of vitamin A as an antioxidant agent on cancer illness by studying its effect before and after the tumor induction regarding to biochemical, histological and molecular genetics levels using male albino mice. Healthy 45 male Swiss albino mice were used. They were randomly divided into nine groups (each with 5 animals). The negative control group, which represents mice did not receive vitamin A or olive oil. Olive oil, which represents mice orally received 0.2214 ml once daily olive oil. O+T+A, O+T+2A, O+T+4A and O+T+O all received 0.2214 ml olive oil for 14 days and then inoculated with EAC cells via intramuscular injection, O+T+A was completed with 0.2214 ml of vitamin A (recommended daily dose), which dissolved in olive oil, O+T+2A was completed with double dose of vitamin A (0.4428 ml), O+T+4A was completed with quadruple dose of vitamin A (0.8856 ml) and O+T+O, was completed with olive oil (0.2214 ml) all for three weeks. A+T+O and A+T+A groups received vitamin A (0.2214 ml) for 14 days, inoculated with EAC cells, then A+T+O group, was completed with olive oil (0.2214 ml) and group A+T+A was completed with vitamin A (0.2214 ml) both for three weeks. Tumor group (T), which inoculated with EAC cells without olive oil or vitamin A treatments. In the biochemical level, increasing of white blood cell count in A+T+A group after only one week compared to T and other vitamin A treated tumor groups was observed, while decrease in hemoglobin levels were observed in T group and vitamin A treated tumor groups. Tumor growth inhibition rate (TGI) results were 33.11, 11.72 and 11.74% for O+T+O, A+T+O and A+T+A groups, respectively. The histological results for the tumor showed some improvement of tumor tissue architecture O+T+O compared to tumor group and all vitamin A tumor treated groups. Change in kidney tissue architecture with presence of tumor cells the kidney in all vitamin A treated tumor groups. In the molecular level, the expression of P16 gene was down-regulated using recommended daily dose of vitamin A in all vitamin A treated groups. P53 gene revealed up-regulation in A+T+O and T groups, while down-regulation was observed in A+T+A and O+T+O groups compared to negative control group. For TERT gene down-regulation was observed due to recommended daily dose of vitamin A in all vitamin A tumor treated groups compared to negative control group. VEGF gene revealed up-regulation in olive oil, O+T+A and A+T+A groups. |
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Supervisor |
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Prof. Dr. Sabah Mahmoud Mohamed Hassan |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1441 AH
2019 AD |
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Added Date |
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Wednesday, October 16, 2019 |
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Researchers
| نسرين أحمد حسنين | Hassanein, Nesreen Ahmed | Researcher | Doctorate | |
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