Document Details
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Document Type |
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Thesis |
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Document Title |
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Genetic and Developmental Studies on the Effect of Performance-Enhancing Drugs in Rattus norvegicus Male دراسات وراثية وتكوينية على تأثير العقاقير المحسنة للأداء في ذكور الجرذان |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Performance-enhancing drugs (PED) are agents used to improve any form of activity performance in humans. The abuse of anabolic androgenic steroids (AASs), the most used types of PED by athletes has been increased rapidly in many countries. Major questions about its use in Saudi community remain unanswered. Hence, the present study was aimed to document the prevalence of PED abuse among a random Saudi population and to study the effect of the most used drug on male fertility regarding to hormonal, histological and molecular genetics levels using male albino rats. A questionnaire containing 31 questions was online distributed and answered by random Saudi population. Eighty animals were used; they were randomly divided into four groups. Each group contains twenty rats. Group I is the negative control group, which represents rats did not receive any treatment, II, III and IV groups were weekly intramuscularly injected with Sustanon®250 by 50, 100 and 150 mg/kg, respectively. Each group was subsequently divided into four subgroups (A, B, C and D) according to the scarifying time (for 4, 8, 12 and 16 weeks). The results of the questionnaire showed that 4.7% of 300 participants admitted using AAS. In male rats, the Sustanon®250 significantly (p< 0.05) increased serum level of testosterone in rats and decreased LH and FSH in almost all experimental subgroups compared to their control subgroups. The histological results of all treated subgroups revealed severe degeneration of the seminiferous tubules, sloughing of spermatogenic cells with necrosis and apoptosis. In the molecular level, the expression of AR, CFTR and INSL3 genes as fertility-related genes in testis tissues were altered due to Sustanon®25 injection. AR gene was mostly down-regulated using all doses in all durations, except for 50 mg/kg dose after eight and 16 weeks, which revealed up-regulation. CFTR gene revealed down-regulation after four and eight weeks using all doses and mostly up-regulated after 12 and 16 weeks using all doses, except for 150 mg/kg dose after 16 weeks, which revealed down-regulation. INSL3 gene mostly down-regulated due to all doses of Sustanon®25, while the up-regulation was observed in 50 and 100 mg/kg doses after 12 and16 weeks, respectively. |
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Supervisor |
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Prof. Sabah Mahmoud Hassan |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Abdulqader Mohammed Omar |
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Added Date |
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Sunday, July 14, 2019 |
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Researchers
| مها هيجان أحمد | Ahmed, Maha Haijan | Researcher | Doctorate | |
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