Document Details

Document Type : Thesis 
Document Title :
Bio-Chemical Studies on Some Species of Saudi Red Sea Seagrasses
دراسات بيو كيميائية على بعض الأنواع من الأعشاب البحرية السعودية بالبحر الأحمر
 
Subject : Faculty of Marine Sciences 
Document Language : Arabic 
Abstract : Red Sea offers an enormous resource of novel compounds and it has been classified as the largest remaining reservoir of natural molecules to be evaluated for drug activity. We have interested to focus our study for searching new medical agents as antioxidants and antitumor agents (Ehrlich ascites carcinoma cells) from sea grasses Halodule uninervis and Thalassia hemprichii. The selected seagrasses were extracted by organic solvents, and partionated by chloroform which saponified by alcoholic KOH. The GC-MS analyses of unsaponified and methylated saponified fractions revealed ~ 50% saturated and ~ 35% unsaturated fatty acids in both seagrasess, where palmitic acid and neophytadiene are the major components in the fractions. A total of seventeen (17) chemical compounds were isolated and identified from the polar fractions of the selected seagrasses extracts. Identifications of the compounds were carried out through spectral analyses. The compounds were identified as nine flavonoids, apigenin, vicenin-2, diosmetin 7-O-β-glucoside, diosmetin 7-O-β-glucoside-2''-sulphate, kaempferol 3-O-β-(6"-E-p-coumaroyl) glucoside, kaempferol 3-O-α-arabinoside, kaempferol 3-O-β-xyloside, quercetin 3-O-β-xyloside, and quercetin 3-O-β-glucoside; two phenolic acids caffeic acid, p-hydroxybenzoic acid; two steroids, β-stigmasterol and β-stigmasterol 3-O-glucoside; fatty acid, palmitic acid; and 4,4'-dihydroxybenzophenone; and nitrogen compound, octopamine. The seagrasses extracts were evaluated for their different biological activities such as antioxidant and antitumor as well as the toxicology effect on the dengue fever vector Aedes aegypti. Thalassia hemprichii was proved to be relatively more effective extract than H. uninervis against mosquito larvae of A. aegypti with LC50 values 190 and 203 ppm, respectively. Seagrasses extracts and their isolated pure compounds have been shown to have various antioxidant activities. Halodule uninervis extract possess high antioxidant effect compared to T. hemprichii and quercetin 3-O-β-glucoside and kaempferol 3-O-α-arabinoside are the active principle at 25 µg/ml, respectively. The total reducing power of kaempferol 3-O-α-arabinoside exhibited the highest total reducing power ability compared to the standard antioxidant (BHT) at 0.45 and 0.463 nm, respectively. The toxicity of both seagrasses extracts and their isolated compounds was examined on Ehrlich ascites carcinoma cell line (EACC). The viable cells were decreased by H. uninervis extract which demonstrated tumoricidal effect with 62% compared to 82% for T. hemprichii extract. Quercetin 3-O-β-glucoside exhibited a moderate antitumor activity; it reduces the viability of EACC to ~ 51% dead cells. The seagrasses extracts were also examined against in vivo Ehrlich ascites carcinoma through estimation of tumor volume and survival time of animals. It was found that the tumor volume of the mice administrated intraperitoneal with 150 mg/Kg of T. hemprichii extract after injection with Ehrlich ascites carcinoma was 11.5 ml and the reduction of tumor volume was found to be 65.66% and slight increase in life span. 
Supervisor : Prof. Dr. Usama Wahid Abd El-Atty Hawas 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2018 AD 
Added Date : Monday, December 10, 2018 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
منير عبدالله بامخرمةBa-makhramah, Munir AbdullahResearcherMaster 

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