Document Details
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Document Type |
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Thesis |
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Document Title |
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Biochemical and clinical applications of microwave-synthesized metal oxides nanostructures تطبيقات كيميائية حيوية وسريرية لتراكيب الأكاسيد المعدنية متناهية الصغر المولفه بالميكروويف |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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The main objective of nanotechnology is to build economical nanotechnologies in various fields. Therefore, Rapid diagnosis of acute myocardial infarction is critical for intervention and treatment. Cardiac biomarkers as AST, LDH1, CK-MB has a low sensitivity for AMI within six hours after an incident and cTnI is better at detecting minor cardiac damage, it was evaluated and this study aimed to design a biosensor for cardiac cTnI and compared the results obtained as done in ELISA. The sensor was designed from golden plate immobilized with anti- troponin I antibody conjugated with HRP enzyme. It was found that, normal troponin I levels (0-15ng/dl) and is chemic heart dieses (15-45 ng/dl) while MI was (>50 ng/dl). Sensitivity of Biosensor was ranged from (85-95%) compared with ELISA technique. This is a simple, stable, and repeatable approach. The modified GNP-GS can be used to detect human cTnI.
On the other hand, metal oxides exist in different forms, stannic (SnO2), stannous (SnO), CeO2 and ZnO. This work describes a new approach for producing these forms in nanoscale dimensions using the chemical microwave assisted route. Fine nanoparticles of metal oxides were formed at the low ratios, whereas others grew at higher ratios in the form of nanosheets and roads. The SEM, TEM, PL, UV-Vis, Raman and XRD results revealed detailed information about the morphological and microstructure properties of these nanostructures and tested as antimicrobial agent against Gram(negative-positive) (Escherichia.coli), (Bacillus.subtilis) bacteria and yeast (Saccharomyces cerevisiae). As shown a SnO, SnO2 (2:20) ratio the most effective against E.coli which causes 81% growth inhibition of E.coli. While SnO, SnO2 at (5:20) causes 82% inhibition for B.subtilis., SnO, SnO2 NS at ratio (8:20) caused (58%) growth inhibition of S. cerevisiae. The best ratios was at 20 μl. While, the inhibition was increased by increasing the CeO2-NPs concentration, reaching to around 70% at a concentration of 20 µL with the molar ratio 5:20 against E.coli. The CeO2-NPs with the ratio 12:20 are the most effective against B.subtilis, which could cause 67% inhibition at 20 µL, but the inhibition was decreased by increasing the CeO2-NPs concentration. On the other hand, CeO2-NPs of the ratio 20:20 causes the highest inhibition for S. cerevisiae, which is about 61% at the low concentration (20 µL). The antimicrobial activity may be attributed to the penetrating power of CeO2-NPs size that generate oxygen species radicals and caused inhibition of bacterial growth. Whereas, the produced ZnO-NS were systematically changed from 25 nm spherical nanoparticles to well-shaped micro size hexagonal nanorods. These ZnO-NS were tested as antimicrobial agent against Gram-negative (E.coli), Gram-positive (B.subtilis) bacteria and yeast (S.cerevisiae). The ZnO-NS with the molar ratio 3:20 had the most effective inhibition against these microbial. The results show 80, 71 and 50% inhibitions of E.coli, B.subtilisand S.cerevisiae, respectively. The best concentration of ZnO-NS for maximum inhibition is found to be 20 µL. |
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Supervisor |
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Dr. Numan Abdullah Salah |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Added Date |
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Thursday, August 24, 2017 |
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Researchers
| وليد محمد الشوافي | ALShawafi, Waleed Mohammad | Researcher | Doctorate | |
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