Document Details
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Document Type |
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Article In Journal |
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Document Title |
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Genetic analysis of the yavapai native americans from west-Central arizona using the illumina MiSeq FGx™ forensic genomics system Genetic analysis of the yavapai native americans from west-Central arizona using the illumina MiSeq FGx™ forensic genomics system |
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Document Language |
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English |
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Abstract |
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Forensically-relevant genetic markers were typed for sixty-two Yavapai Native Americans using the
ForenSeqTM DNA Signature Prep Kit.These data are invaluable to the human identity community due to
the greater genetic differentiation among Native American tribes than among other subdivisions within
major populations of the United States. Autosomal, X-chromosomal, and Y-chromosomal short tandem
repeat (STR) and identity-informative (iSNPs), ancestry-informative (aSNPs), and phenotype-informative
(pSNPs) single nucleotide polymorphism (SNP) allele frequencies are reported. Sequence-based allelic
variants were observed in 13 autosomal, 3 X, and 3 Y STRs. These observations increased observed and
expected heterozygosities for autosomal STRs by 0.081
0.068 and 0.073
0.063, respectively, and
decreased single-locus random match probabilities by 0.051
0.043 for 13 autosomal STRs. The
autosomal random match probabilities (RMPs) were 2.37
10–26 and 2.81
10–29 for length-based and
sequence-based alleles, respectively. There were 22 and 25 unique Y-STR haplotypes among 26 males,
generating haplotype diversities of 0.95 and 0.96, for length-based and sequencebased alleles,
respectively. Of the 26 haplotypes generated,17 were assigned to haplogroup Q, three to haplogroup R1b,
two each to haplogroups E1b1b and L, and one each to haplogroups R1a and I1. Male and female
sequence-based X-STR random match probabilities were 3.28
10–7 and 1.22
10–6, respectively. The
average observed and expected heterozygosities for 94 iSNPs were 0.39
0.12 and 0.39
0.13,
respectively, and the combined iSNP RMP was 1.08
10–32. The combined STR and iSNP RMPs were
2.55
10–58 and 3.02
10–61 for length-based and sequence-based STR alleles, respectively. Ancestry
and phenotypic SNP information, performed using the ForenSeqTM Universal Analysis Software,
predicted black hair, brown eyes, and some probability of East Asian ancestry for all but one sample that
clustered between European and Admixed American ancestry on a principal components analysis. These
data serve as the
first population assessment using the ForenSeqTM panel and highlight the value of
employing sequence-based alleles for forensic DNA typing to increase heterozygosity, which is beneficial
for identity testing in populations with reduced genetic diversity. |
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ISSN |
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1872-4973 |
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Journal Name |
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Forensic Science International: Genetics |
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Volume |
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24 |
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Issue Number |
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1 |
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Publishing Year |
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1437 AH
2016 AD |
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Article Type |
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Article |
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Added Date |
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Wednesday, July 12, 2017 |
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Researchers
| Frank R. Wendt | Wendt, Frank R. | Researcher | Doctorate | |
| Jennifer D. Churchill | Churchill, Jennifer D. | Researcher | Doctorate | |
| Nicole M. Novroski | Novroski, Nicole M. | Researcher | Doctorate | |
| Jonathan L. King | King, Jonathan L. | Researcher | Doctorate | |
| Jillian Ng | Ng, Jillian | Researcher | Doctorate | |
| Robert F. Oldt | Oldt, Robert F. | Researcher | Doctorate | |
| Kelly L. McCulloh | McCulloh, Kelly L. | Researcher | Doctorate | |
| Jessica A. Weise | Weise, Jessica A. | Researcher | Doctorate | |
| David Glenn Smith | Smith, David Glenn | Researcher | Doctorate | |
| Sreetharan Kanthaswamy | Kanthaswamy, Sreetharan | Researcher | Doctorate | |
| Bruce Budowle | Budowle, Bruce | Researcher | Doctorate | |
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