Document Details Document Type : Article In Journal  Document Title : A short route of covalent biofunctionaliztion silicon surfaces طريقة مختصرة للتوظيف الحيوي لأسطح السيليكون   Subject : Chemical & materials engineering  Document Language : English  Abstract : Covalently attached organic monolayers on etched Si(1 1 1) surfaces were prepared by heating solutions of 1-alkenes and 1-alkynes in a refluxing mesitylene. Surface modification was monitored by measurement of the static water contact angle, X-ray photoelectron spectroscopy (XPS), infrared reflection absorption spectroscopy (IRRAS), and atomic force microscopy (AFM). Flat and clean N-hydroxysuccinimide (NHS)-ester-terminated/1-decyl mixed monolayers were covalently attached in one step onto a silicon surface. This procedure allows a mild and rapid functionalization of the surface by substitution of the NHS-ester moieties with amines at room temperature. The NHS-ester groups were shown to be fully intact onto the surface. The surface reactivity of the NHS-ester moieties toward amines was qualitatively and quantitatively evaluated via the reaction with methoxytetraethyleneglycolamine (TEGamine) and finally functionalized with single strand and complete DNA molecules. Moreover, domains of DNA were selectively immobilized, on silicon surface making use of TEGamine, which acts as protein repelling agent and therefore prevented non-specific DNA adsorption. The resulting DNA-modified surfaces have shown excellent specificity, and chemical and thermal stability under hybridization conditions.  ISSN : 09254005  Journal Name : Sensors and Actuators, B: Chemical  Volume : 152  Issue Number : 2  Publishing Year : 1432 AH 2011 AD  Article Type : Article  Added Date : Tuesday, April 12, 2011  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email أحمد عرفات خميس Kamis, Ahmed Arafat Investigator Doctorate akhamis@kau.edu.sa محمد عبدالرحمن داعوس Daous, Muhammad Abdul Rahman Researcher Doctorate mdaous@kau.edu.sa Files File Name Type Description  29613.pdf pdf A short route of covalent biofunctionaliztion silicon surfaces Back To Researches Page