Document Details

Document Type : Article In Journal 
Document Title :
Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution
تركيبة جديده صبغة سّيانين ال;-نافثوثيازول مونوميثاين لكشف الدي.إن.إيه في المحلول المائيّ
 
Subject : Synthesis of a new β-naphthothiazole monomethine cyanine dye for the detection of DNA in aqueous solution 
Document Language : English 
Abstract : Novel monomethine cyanine dye (MC) derived from β-naphthothiazole and benzothiazole has been prepared and characterized by 1H and 13C NMR, FTIR, ESIMS, elemental analyses, absorption and fluorescence spectroscopy. The dye was conveniently synthesized by the condensation of two sulfate heterocyclic quaternary salts. The interaction between calf thymus DNA (ct-DNA) in tris(hydroxymethyl)aminomethane–HCl (Tris–HCl) aqueous buffer solution and MC has been studied with spectral fluorescence method. The binding constant value has been determined by fluorescence titration of MC with ct-DNA concentrations. The result obtained is consistent with an intercalative binding interaction between MC and ct-DNA. Compared with ethidium bromide (EB), MC showed a huge fluorescence enhancement upon mixing with ct-DNA 
ISSN : 1012-1319 
Journal Name : Science Journal 
Volume : 75 
Issue Number : 5 
Publishing Year : 1431 AH
2010 AD 
Article Type : Article 
Added Date : Sunday, February 6, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبدالله عسيريAsiri, Abdullah ResearcherDoctorate 

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