Document Details Document Type : Article In Journal  Document Title : FIBROBLAST CELLS IN TISSUE CULTURE OF THE INTRAARTICULAR FIBROBLAST CELLS IN TISSUE CULTURE OF THE INTRAARTICULAR   Document Language : English  Abstract : Fibroblast cells are sensitive to their normal extracellular matrix containing so many bioactive molecules and collagen fi- bers, as well as other cells. An in-vivo like system was established to study the behaviour of fibroblasts in-vitro when cul-tured together with the surrounding extracellular substratum. Thirty intra-articular disks of the craniomandibular joints were surgically obtained from 15 young adult albino rats, then 26 were cultured in a chemically defined medium for different per- iods, and the other 4 disks were used as controls. Examination of sections obtained from these disks revealed that, the pro- liferation of fibroblasts started at about the 4th day in culture, and reached maximum by the 7th day. This proliferation was keeping pace with differentiation and the cells formed a confluent monolayer with typical cobblestone appearance. On the 10th and 14th days, the fibroblasts were gradually dedifferentiating and exhibited different degrees of smashing up and break down. It can be concluded that this system is an excellent model for culturing and obtaining the fibroblast cells till 7 days to be used for many applications. Also, some modifications can be introduced to the system to improve its validity to culture the fibroblast cells for longer periods. Assistant Professor, Oral Biology Department, Faculty of Dentistry, Alexandria University.  ISSN : 0  Journal Name : Cairo Dental. Journal  Volume : 13  Issue Number : 3  Publishing Year : 1997 AH 1997 AD  Article Type : Article  Added Date : Monday, May 5, 2008  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email سامية عمر omar, samia Investigator Doctorate ssomar@kau.edu.sa Files File Name Type Description  FIBROBLAST CELLS IN TISSUE CULTURE OF THE INTRA.pdf pdf   Back To Researches Page